To investigate the Anxiolytic effect of P. pinnata leaf extract in mice. The Anxiolytic effect of P. pinnata extract in mice was studied on acute and chronic treatment. On acute administration, effect of extract was observed 30 minutes after treatment and in chronic treatment the animals were treated with extract and diazepam for 14 days. After the treatment animals were observed for locomotor activity in Actophotometer, behavioral effect was studied in open field test, anxiolytic and anxiogenic effects were studied in elevated plus maize test and compared with control group, also the brain 5-HT concentrations was estimated. On acute administration, the extract showed significant dose dependent decrease in locomotor activity. While on chronic administration, both diazepam (0.5mg/kg) and the extract showed significant increase in the locomotor activity. Diazepam 0.25mg/kg on acute administration showed significant changes in open field test. Also significant changes in parameters of elevated plus maze test were observed. While diazepam 0.5mg/kg and the extract showed significant changes but the mice were more depressed. After chronic administration of diazepam 0.5mg/kg and P. pinnata extract, both showed significant changes in open field test and elevated plus maze when compared with control group. On acute administration 5-HT level got decreased significantly by the extract as well as diazepam. The ethanolic extract of P. pinnata showed significant anxiolytic activity comparable with diazepam.
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